Publications
2009
Hacker, D.L., De Jesus, M., Wurm, F. M.
Review Paper: 25 Years of Recombinant Proteins from Reactor-Grown Cells — Where Do We Go From Here? , Biotechnology Advances, October 2009, Elsevier,PDFversion
Matasci, M., Hacker, D.L., Wurm, F. M.
Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects, Drug Discovery Today
Medium and process development for high yield, high-density suspension cultures : From low throughput spinner flasks to high throughput milliliter reactors Bioprocess International, Vol 7, Suppl. 1, February 2009, p13- p19
Chenuet, S., Derouazi, M., Hacker, D. and Wurm, F.
DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cells Lines. Methods Mol. Biol. 518, 1-14.
Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1000 L. Biochemical Eng. J. doi: 10.1016/j.bej.2009.02.003
2008
Backliwal G, Hildinger M, Chenuet S, Dejesus M, Wurm F.M.
Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells.
N Biotechnol. 2008;25(2-3):162-6. Epub 2008 Aug 27.
Chenuet S, Martinet D, Besuchet-Schmutz N, Wicht M, Jaccard N, Bon AC,
Derouazi M, Hacker DL, Beckmann JS, Wurm FM.
Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.
Biotechnol Bioeng. 2008 Dec 1;101(5):937-45.
Backliwal G, Hildinger M, Chenuet S, Wulhfard S, De Jesus M, Wurm F.M.
Rational vector design and multi-pathway modulation of HEK 293E cells yield
recombinant antibody titers exceeding 1 g/l by transient transfection under
serum-free conditions.
Nucleic Acids Res. 2008 Sep;36(15):e96. Epub 2008 Jul 10.
Bertschinger M, Schertenleib A, Cevey J, Hacker DL, Wurm F.M.
The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese Hamster Ovary cells.
Mol Biotechnol. 2008 Oct;40(2):136-43. Epub 2008 Jun 10.
Zhang X, Stettler M, Reif O, Kocourek A, De Jesus M, Hacker DL, Wurm F.M.
Shaken helical track bioreactors: Providing oxygen to high-density cultures of
mammalian cells at volumes up to 1000 L by surface aeration with air.
N Biotechnol. 2008;25(1):68-75. Epub 2008 May 19.
Backliwal G, Hildinger M, Kuettel I, Delegrange F, Hacker DL, Wurm FM.
Valproic acid: a viable alternative to sodium butyrate for enhancing protein
expression in mammalian cell cultures.
Biotechnol Bioeng. 2008 Sep 1;101(1):182-9.
Wulhfard S, Tissot S, Bouchet S, Cevey J, De Jesus M, Hacker DL, Wurm F.M.
Mild hypothermia improves transient gene expression yields several fold in
Chinese hamster ovary cells.
Biotechnol Prog. 2008 Mar-Apr;24(2):458-65. Epub 2008 Jan 26.
Wurm F.M.
Manufacturing of biopharmaceuticals and implications for biosimilars.
Kidney Blood Press Res. 2007;30 Suppl 1:6-8. Epub 2007 Aug 27. Review.
Stettler M, Zhang X, Hacker DL, De Jesus M, Wurm F.M.
Novel orbital shake bioreactors for transient production of CHO derived IgGs.
Biotechnol Prog. 2007 Nov-Dec;23(6):1340-6. Epub 2007 Oct 3.
Backliwal G, Hildinger M, Hasija V, Wurm F.M.
High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.
Biotechnol Bioeng. 2008 Feb 15;99(3):721-7.
K. Stirnemann, J.F. Romero, L. Baldi, B. Robert, V. Cesson, G.S. Besra, M. Zauderer, F.M. Wurm, G. Corrradin, J.P. Mach, H.R. MacDonald and A. Donda.
Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice.
J. Clin. Invest., Vol. 118, Nr. 3, pp. 994-1005, 2008.
2007
M. Hildinger, L. Baldi, M. Stettler and Wurm F.M.
High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells.
Biotechnol Lett, 2007.
N. Muller, M. Derouazi, F. Van Tilborgh, S. Wulhfard, D.L. Hacker, M. Jordan and Wurm F.M.
Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems.
Biotechnol Lett, Vol. 29, Nr. 5, pp. 703-11, 2007.
L. Baldi, D.L. Hacker, M. Adam and Wurm F.M.
Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives.
Biotechnology Letters, Vol. 29, Nr. 5, pp. 677-84, 2007.
K. Ehrmann, N. Saillen, F. Vincent, M. Stettler, M. Jordan, Wurm F.M., P.A. Besse and R. Popovic.
Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells.
Lab on a chip, Vol. 7, Nr. 3, pp. 373-80, 2007.
K. Ehrmann, K. Pataky, M. Stettler, Wurm F.M., J. Brugger, P.A. Besse and R. Popovic.
NMR spectroscopy and perfusion of mammalian cells using surface microprobes
Lab on a Chip, Vol. 7, Nr. 3, pp. 381-383, 2007.
2006
M. Stettler, N. Jaccard, D. Hacker, M. De Jesus, Wurm F.M. and M. Jordan.
New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells.
Biotechnol Bioeng, Vol. 95, Nr. 6, pp. 1228-33, 2006.
M. Bertschinger, G. Backliwal, A. Schertenleib, M. Jordan, D.L. Hacker and Wurm F.M.,
Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery.
J Control Release, Vol. 116, Nr. 1, pp. 96-104, 2006.
F. Grosjean, M. Bertschinger, D.L. Hacker and Wurm F.M.
Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.
Biotechnology letters, Vol. 28, Nr. 22, pp. 1827-33, 2006.
M. Derouazi, D. Martinet, N. Besuchet Schmutz, R. Flaction, M. Wicht, M. Bertschinger, D.L. Hacker, J.S. Beckmann and F.M. Wurm F.M.
Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.
Biochemical and biophysical research communications, Vol. 340, Nr. 4, pp. 1069-77, 2006.
M. Derouazi, R. Flaction, P. Girard, M. de Jesus, M. Jordan and F.M. Wurm F.M.
Generation of recombinant Chinese hamster ovary cell lines by microinjection.
Biotechnology letters, Vol. 28, Nr. 6, pp. 373-82, 2006.
M. Bertschinger, C. Burki, G. Backliwal, D.L. Hacker, M. Jordan and Wurm F.M.
Polyethylenimine-based quality control assay for plasmid DNA.
Analytical biochemistry, Vol. 356, Nr. 2, pp. 309-11, 2006.
