Publications

Learn more about Prof. Florian Wurm’s and Dr. Maria de Jesus’ scientific discoveries that paved the way for ExcellGene’s scientific foundation.

White Papers are your gateway to stay tuned with challenges and trends facing the biomanufacturing industry, here from the scientific leaders and founders of ExcellGene, Professor Florian M. Wurm and Dr. Maria J. Wurm (AKA Maria De Jesus). Both are highly respected and frequently requested individuals (accumulated experience in the industry of 6 decades) for scientific and technological advice on the manufacture of recombinant biopharmaceuticals under large scale operation. 


 

  • F.M. Wurm and M.J. Wurm (2017): Cloning of CHO Cells, Productivity and Genetic Stability – A discussion, Processes, 5, 20, doi:10.3390/pr5020020
    http://www.mdpi.com/2227-9717/5/2/20
  • Wurm, F.M. (2014): CHO history, CHO Evolution and CHO Genomics – an unsolvable Enigma? Hauser and Wagner: Animal Cell Biotechnology De Gruyter. Pp. 38-60
  • Wurm, F.M. (2013): CHO Quasispecies – Implication for Manufacturing Processes Processes 1,3, 296-311
    http://www.mdpi.com/2227-9717/1/3/296.
  • F.M. Wurm (2010), commentary: Deciphering the Burgess Shale of single-use bioreactors Next Generation Pharmaceutical Sunday, Nov. 14, 2010 
    http://www.ngpharma.eu.com/article/Deciphering-the-Burgess-Shale-of-single-use-bioreactors/
  • De Jesus, M., Wurm, F.M. (2009): Medium and process development for high yield, high-density suspension cultures: From low throughput Spinner flasks to high throughput milliliter reactors Bioprocess International, Vol 7, Suppl. 1,  February 2009, p13- p19
  • D.L. Hacker, M. De Jesus, F.M. Wurm (2009): 25 years of recombinant proteins from reactor-grown cells : where do we go from here? Biotechnol Adv (2009), doi:10.1016/j.biotechadv.2009.05.008
  • Wurm, F. M. (2007): Manufacturing of biopharmaceuticals and implications for biosimilars Kidney Blood Press Res. Vol. 30 Suppl 1, pp. 6-8.
  • Wurm, F.M. (2004): Production of recombinant protein therapeutics in cultivated mammalian cells Nature Biotechnology 22, 11, 1393-1398.

  • Wurm, F.M., .K.A. Gwinn, and R. E. Kingston (1986):
    Inducible overexpression of the mouse c-myc protein in mammalian cells
    Proc Natl Acad Sci USA : 83(15), pp. 5414-5418
  • Pallavicini, M .G., DeTeresa, P.S. and Wurm, F.M. (1989):
    Use of fluorescence in situ hybridisation to detect and monitor transfected and amplified sequences in recombinant CHO cells
    Dev. Biol. Stand., vol. 70, pp. 165-172
  • Capon, D.C., Chamow, S., Mordenti, J., Marsters, S.A., Gregory, T., Mitsuya, H., Byrn, R.A., Lucas, C. , Wurm, F.M., Groopman, J.E., Broder S. and Smith D.H. (1989):
    Designing Immunoadhesins for AIDS Therapy
    Nature 337 (6207) pp. 525-531
  • Wurm, F.M. (1990):
    Review – Integration, amplification and stability of plasmid sequences in CHO cell cultures
    Biologicals 18, pp.159-164.
  • Wurm, F.M. (1990):
    Integration, amplification and stability of plasmid sequences in CHO cell cultures
    Biologicals 18(3): 159-64
  • Anderson, K.P., Lie, Y.F., Low, M.L., Williams, S.R., Fennie, E.H., Nguyen, T.P. and Wurm, F.M. (1990):
    Presence and transcription of intracisternal a particle related sequences in CHO cells
    J. Virol, 64 (5), 2021-2032
  • Pallavicini, M.G., DeTeresa, P.S., Rosette, C., Gray, J.W. and Wurm, F.M. (1990):
    Effects of Methotrexate (MTX) on Transfected DNA Stability in Mammalian Cells
    Mol Cell Biol, 10 (1) pp. 401-404
  • Bennett, W.F., Paoni, N.F., Keyt, B.A., Botstein, D., Jones, A.J.S., Presta, L., Wurm, F.M., Zoller, M.J. (1991):
    High resolution analysis of functional determinants on human tissue-type plasminogen-activator
    J. Biol Chem 226 (8), pp.5191-5201
  • Wurm, F.M. (1994):
    Characterisation of recombinant CHO cells
    Cell and Tissue Culture: Laboratory Procedures, J.B. Griffiths, A. Doyle, D.G. Newell, Eds., Wiley Interscience 1994.
  • Wurm, F.M. and Petropoulos, C.P. (1994):
    Plasmid integration, amplification and cytogenetics in CHO cells
    Questions and comments. Biologicals 22, pp. 95-102
  • Wurm, F.M., Johnson, A., Ryll, T., Köhne, C., Scherthan, H., Glaab, F., Lie, Y.S., Petropoulos C.J and Arathoon, W.R. (1996):
    Gene transfer and amplification in CHO cells – efficient methods for maximising specific productivity and assessment of genetic consequences
    Annals. N.Y. Acad. Sci. 782, pp 70-78
  • Jordan, M., Schallhorn, A. and Wurm, F.M. (1996):
    Transfecting mammalian cells: optimisation of critical parameters affecting calcium-phosphate precipitate formation
    Nucleic Acids Res 24(4), pp. 596-601
  • Wurm, F.M. (1997):
    Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells
    Genetic Manipulation of Mammalian Cells. Hj. Hauser, R. Wagner, Eds. Walter de Gruyter, pp. 87-120
  • Jordan, M., Köhne, C. and Wurm, F.M. (1997):
    Principles of a scaleable transient transfection and expression technology for mammalian cells
    M.J. Carrondo, B. Griffiths and J.P. Moreira: Animal Cell Technology – from Vaccines to Genetic Medicine, pp. 47-50. Kluwer Academic Publishers
  • Wurm, F.M. and Schiffman, D. (1998):
    Cytogenetic characterisation of recombinant cells
    Methods in Molecular Biology – Mammalian Cell Biology. Nigel Jenkins, Ed. Humana press, pp. 49-59
  • Wurm, F.M., Ashkenazi, A., Chamow, S. (1998):
    Optimizing Production and Recovery of Immunoadhesins
    Antibody Fusion Proteins. S. Chamow and A. Ashkenazi, Eds. Wiley and Sons, pp. 281-308
  • De Jesus, M., Bourgeois, M., Jordan, M., Zahn, M., Mermod, N., Amstutz, H. and Wurm, F.M. (1999):
    Establishing and developing CHO cell lines for commercial production of recombinant human anti Rh D antibodies
    Animal Cell Technology – Products from Cells, Cells as Products, A Bernard, B Griffiths, W Noe, F Wurm, Eds., Kluwer Acad. Publishers, Dordrecht, pp. 91-94
  • Wurm, F.M. and Bernard, A. (2001):
    Transient gene expression from mammalian cells – a new chapter in animal cell technology?
    Cytotechnology 35, 155-156
  • Wurm, F.M. (2004):
    Production of recombinant protein therapeutics in cultivated mammalian cells
    Nature Biotechnology 22, 11, 1393-1398
  • M. Jordan and F. Wurm, (2004):
    Transfection of adherent and suspended cells by calcium phosphate
    Methods, Vol. 33, Nr. 2, pp. 136-43
  • De Jesus, M., Girard, P., Bourgeois, Baumgartner, M., Jacko, G., Amstutz, H. and Wurm, F.M. (2004):
    Tubespin satellites: a fast track approach for process development with animal cells using shaking technology
    Biochemical Engineering Journal 17 (3), pp. 217-223
  • Muller, N., Girard, P., Jordan, M., Wurm, F.M. (2005):
    Orbital shaker technology for the cultivation of mammalian cells in suspension
    Biotechnol. Bioeng. 89 (4), pp. 400-406
  • Reclari, M, Dreyer, M., Tissot, S., Obreschkow, D., Wurm, F.M., Farhat, M. (2014):
    Surface wave dynamics in orbital shaken cylindrical containers
    Physics of Fluids 26, 052104, doi. 10.1063/1.4874612

White Papers are your gateway to stay tuned with challenges and trends facing the biomanufacturing industry, here from the scientific leaders and founders of ExcellGene, Professor Florian M. Wurm and Dr. Maria J. Wurm (AKA Maria De Jesus). Both are highly respected and frequently requested individuals (accumulated experience in the industry of 6 decades) for scientific and technological advice on the manufacture of recombinant biopharmaceuticals under large scale operation. 


 

  • F.M. Wurm and M.J. Wurm (2017): Cloning of CHO Cells, Productivity and Genetic Stability – A discussion, Processes, 5, 20, doi:10.3390/pr5020020
  • Wurm, F.M. (2014): CHO history, CHO Evolution and CHO Genomics – an unsolvable Enigma? Hauser and Wagner: Animal Cell Biotechnology De Gruyter. Pp. 38-60
  • Wurm, F.M. (2013): CHO Quasispecies – Implication for Manufacturing Processes Processes 1,3, 296-311 http://www.mdpi.com/2227-9717/1/3/296.
  • F.M. Wurm (2010), commentary: Deciphering the Burgess Shale of single-use bioreactors Next Generation Pharmaceutical Sunday, Nov. 14, 2010 http://www.ngpharma.eu.com/article/Deciphering-the-Burgess-Shale-of-single-use-bioreactors/
  • De Jesus, M., Wurm, F.M. (2009): Medium and process development for high yield, high-density suspension cultures: From low throughput Spinner flasks to high throughput milliliter reactors Bioprocess International, Vol 7, Suppl. 1,  February 2009, p13- p19
  • D.L. Hacker, M. De Jesus, F.M. Wurm (2009): 25 years of recombinant proteins from reactor-grown cells : where do we go from here? Biotechnol Adv (2009), doi:10.1016/j.biotechadv.2009.05.008
  • Wurm, F. M. (2007): Manufacturing of biopharmaceuticals and implications for biosimilars Kidney Blood Press Res. Vol. 30 Suppl 1, pp. 6-8.
  • Wurm, F.M. (2004): Production of recombinant protein therapeutics in cultivated mammalian cells Nature Biotechnology 22, 11, 1393-1398.

  • Wurm, F.M., .K.A. Gwinn, and R. E. Kingston (1986):
    Inducible overexpression of the mouse c-myc protein in mammalian cells
    Proc Natl Acad Sci USA : 83(15), pp. 5414-5418
  • Pallavicini, M .G., DeTeresa, P.S. and Wurm, F.M. (1989):
    Use of fluorescence in situ hybridisation to detect and monitor transfected and amplified sequences in recombinant CHO cells
    Dev. Biol. Stand., vol. 70, pp. 165-172
  • Capon, D.C., Chamow, S., Mordenti, J., Marsters, S.A., Gregory, T., Mitsuya, H., Byrn, R.A., Lucas, C. , Wurm, F.M., Groopman, J.E., Broder S. and Smith D.H. (1989):
    Designing Immunoadhesins for AIDS Therapy
    Nature 337 (6207) pp. 525-531
  • Wurm, F.M. (1990):
    Review – Integration, amplification and stability of plasmid sequences in CHO cell cultures
    Biologicals 18, pp.159-164.
  • Wurm, F.M. (1990):
    Integration, amplification and stability of plasmid sequences in CHO cell cultures
    Biologicals 18(3): 159-64
  • Anderson, K.P., Lie, Y.F., Low, M.L., Williams, S.R., Fennie, E.H., Nguyen, T.P. and Wurm, F.M. (1990):
    Presence and transcription of intracisternal a particle related sequences in CHO cells
    J. Virol, 64 (5), 2021-2032
  • Pallavicini, M.G., DeTeresa, P.S., Rosette, C., Gray, J.W. and Wurm, F.M. (1990):
    Effects of Methotrexate (MTX) on Transfected DNA Stability in Mammalian Cells
    Mol Cell Biol, 10 (1) pp. 401-404
  • Bennett, W.F., Paoni, N.F., Keyt, B.A., Botstein, D., Jones, A.J.S., Presta, L., Wurm, F.M., Zoller, M.J. (1991):
    High resolution analysis of functional determinants on human tissue-type plasminogen-activator
    J. Biol Chem 226 (8), pp.5191-5201
  • Wurm, F.M. (1994):
    Characterisation of recombinant CHO cells
    Cell and Tissue Culture: Laboratory Procedures, J.B. Griffiths, A. Doyle, D.G. Newell, Eds., Wiley Interscience 1994.
  • Wurm, F.M. and Petropoulos, C.P. (1994):
    Plasmid integration, amplification and cytogenetics in CHO cells
    Questions and comments. Biologicals 22, pp. 95-102
  • Wurm, F.M., Johnson, A., Ryll, T., Köhne, C., Scherthan, H., Glaab, F., Lie, Y.S., Petropoulos C.J and Arathoon, W.R. (1996):
    Gene transfer and amplification in CHO cells – efficient methods for maximising specific productivity and assessment of genetic consequences
    Annals. N.Y. Acad. Sci. 782, pp 70-78
  • Jordan, M., Schallhorn, A. and Wurm, F.M. (1996):
    Transfecting mammalian cells: optimisation of critical parameters affecting calcium-phosphate precipitate formation
    Nucleic Acids Res 24(4), pp. 596-601
  • Wurm, F.M. (1997):
    Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells
    Genetic Manipulation of Mammalian Cells. Hj. Hauser, R. Wagner, Eds. Walter de Gruyter, pp. 87-120
  • Jordan, M., Köhne, C. and Wurm, F.M. (1997):
    Principles of a scaleable transient transfection and expression technology for mammalian cells
    M.J. Carrondo, B. Griffiths and J.P. Moreira: Animal Cell Technology – from Vaccines to Genetic Medicine, pp. 47-50. Kluwer Academic Publishers
  • Wurm, F.M. and Schiffman, D. (1998):
    Cytogenetic characterisation of recombinant cells
    Methods in Molecular Biology – Mammalian Cell Biology. Nigel Jenkins, Ed. Humana press, pp. 49-59
  • Wurm, F.M., Ashkenazi, A., Chamow, S. (1998):
    Optimizing Production and Recovery of Immunoadhesins
    Antibody Fusion Proteins. S. Chamow and A. Ashkenazi, Eds. Wiley and Sons, pp. 281-308
  • De Jesus, M., Bourgeois, M., Jordan, M., Zahn, M., Mermod, N., Amstutz, H. and Wurm, F.M. (1999):
    Establishing and developing CHO cell lines for commercial production of recombinant human anti Rh D antibodies
    Animal Cell Technology – Products from Cells, Cells as Products, A Bernard, B Griffiths, W Noe, F Wurm, Eds., Kluwer Acad. Publishers, Dordrecht, pp. 91-94
  • Wurm, F.M. and Bernard, A. (2001):
    Transient gene expression from mammalian cells – a new chapter in animal cell technology?
    Cytotechnology 35, 155-156
  • Wurm, F.M. (2004):
    Production of recombinant protein therapeutics in cultivated mammalian cells
    Nature Biotechnology 22, 11, 1393-1398
  • M. Jordan and F. Wurm, (2004):
    Transfection of adherent and suspended cells by calcium phosphate
    Methods, Vol. 33, Nr. 2, pp. 136-43
  • De Jesus, M., Girard, P., Bourgeois, Baumgartner, M., Jacko, G., Amstutz, H. and Wurm, F.M. (2004):
    Tubespin satellites: a fast track approach for process development with animal cells using shaking technology
    Biochemical Engineering Journal 17 (3), pp. 217-223
  • Muller, N., Girard, P., Jordan, M., Wurm, F.M. (2005):
    Orbital shaker technology for the cultivation of mammalian cells in suspension
    Biotechnol. Bioeng. 89 (4), pp. 400-406
  • Reclari, M, Dreyer, M., Tissot, S., Obreschkow, D., Wurm, F.M., Farhat, M. (2014):
    Surface wave dynamics in orbital shaken cylindrical containers
    Physics of Fluids 26, 052104, doi. 10.1063/1.4874612

Let's work together.

Whitepapers

Your gateway to stay tuned with challenges and trends facing the biomanufacturing industry, all from the perspective of the renowned Prof. Florian M. Wurm.  F.M. Wurm is internationally recognized leader of CHO technologies and the manufacture of proteins at large scale for pharmaceutical applications.

List of whitepapers

  • Wurm, F.M.   (2004): Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 22, 11, 1393-1398.
  • Wurm, F. M. (2007) Manufacturing of biopharmaceuticals and implications for biosimilars, Kidney Blood Press Res. Vol. 30 Suppl 1, pp. 6-8.
  • De Jesus, M., Wurm, F.M. (2009) Medium and process development for high yield, high-density suspension cultures: From low throughput Spinner flasks to high throughput milliliter reactors Bioprocess International, Vol 7, Suppl. 1,  February 2009, p13- p19
  • D.L. Hacker, M. De Jesus, F.M. Wurm (2009) : 25 years of recombinant proteins from reactor-grown cells : where do we go from here ? Biotechnol Adv (2009), doi:10.1016/j.biotechadv.2009.05.008
  • F.M. Wurm (2010): Commentary: Deciphering the Burgess Shale of single-use bioreactors in : Next Generation Pharmaceutical Sunday, Nov. 14, 2010 http://www.ngpharma.eu.com/article/Deciphering-the-Burgess-Shale-of-single-use-bioreactors/
  • Wurm, F.M. (2014) CHO history, CHO Evolution and CHO Genomics – an unsolvable Enigma? In. Hauser and Wagner: Animal Cell Biotechnology De Gruyter. Pp. 38-60
  • Wurm, F.M. (2013) CHO Quasispecies – Implication for Manufacturing Processes. Processes 1,3, 296-311 To be downloaded at: http://www.mdpi.com/2227-9717/1/3/296.

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