Everything from DNA vector to purified protein.
ExcellGene covers the value chain from DNA construction & transfection, to scalable process development, to rapid protein manufacture.
Due to our science-based focus, we ensure some of the fastest and most reliable results that the industry can provide.
Cell line development
From DNA to high-yielding clonal population and process
What we do
- Upstream and Downstream Process Development
- Stable cell line within 12 months
- Typically, 10x increases in titer
- Up to 40 media, 20 feed types screened in parallel
- Customized media optimization
- Thorough analytics throughout USP and DSP.
ExcellGene’s expertise in Upstream Process Development is defined by years of work with thousands of proteins. We work with many cell lines including CHO, HEK-293, SP2.0, and NSO.
Cell line generation – a stable, high-yielding cell line within 12 months. An ExcellGene cell host has never failed a stability test. Bulletproof clonality.
Cell line optimization – typically, 10x increases in titer.
Extensive media and feed screening – up to 40 media and 20 feed types in parallel. We also develop customer-owned media and feed formulations.
Thorough analytics – throughout USP and DSP.
A dedicated project leader with years of experience will guide the efforts for your project.
Rapid protein production
Rapid production in weeks from the 1mL to 10L scale
What we do
- Fast, reliable protein production
- Research & development-grade
- Feasability studies
- High-throughput screening of protein variants
- Your product within 4 weeks.
We provide fast, reliable protein production services.
Over decades, our proprietary CHOExpress™ and HEKExpress-293™ cells have been optimized for rapid growth, efficient DNA uptake, high protein expression and high density in suspension cultures.
Upon protein sequence receipt, we perform vector and DNA sequence optimization to obtain high titers after transfections.
In addition, our CHOExpress™ expression system allows screening of protein variants in record time: up to 1000 protein variants tested in parallel and up to 80µg per protein generated.
The bottom line: extremely rapid production from the 1mL to 10L scale, and the delivery of your product within 4 weeks after vector availability.
cGMP Cell Banking
Master cell Banks and working cell banks of
- up to 500 vials each can be prepared,
- typically with over 10 million cells per vial and
- thawing at viabilities of over 95%.
Our flexible cGMP unit allows banking of cell cultures for any client to use an immortalized cell line for protein production and more.
A separate DSP unit includes single use columns for the purification of biological material.
ISO5 (EU class A, US 100) and ISO7 (EU class C, US 10’000).
Our clean room installation has been inspected and approved by the Swiss Agency for Therapeutic Products Swissmedic.