Knowledge Base
Welcome to the lab.
Learn more about the discoveries that paved the way for ExcellGene’s scientific foundation. Videos, White Papers and publications, (co-) authored by F. M. Wurm, M.J. Wurm (De Jesus) and ExcellGene scientists over three decades.
Videos White Papers PublicationsVideos
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From DNA to Therapeutic Protein in CHO cells
In 1957, a sample of cells from the ovary of a Chinese hamster was cultured and described. Unknowingly, the scientists establishing the Chinese Hamster Ovary (CHO) cells had kicked off a revolution within the field of biotech and biosynthesis of therapeutic proteins.
White Papers
- Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens (Summary) 2020 Processes, 8(12), 1539; doi.org/10.3390/pr8121539
- Cloning of CHO Cells, Productivity and Genetic Stability – a Discussion 2017 Processes, 5, 20, doi:10.3390/pr5020020
- CHO History, CHO Evolution and CHO Genomics – an Unsolvable Enigma? 2014 Hauser and Wagner: Animal Cell Biotechnology De Gruyter. Pp. 38-60
- CHO Quasispecies – Implication for Manufacturing Processes 2013 Processes 1,3, 296-311
- Commentary: Deciphering the Burgess Shale of Single-use Bioreactors 2010 Next Generation Pharmaceutical
- Medium and Process Development for High Yield, High-Density Suspension Cultures: from Low Throughput Spinner flasks to High Throughput Milliliter Reactors 2009 Bioprocess International, Vol 7, Suppl. 1, p.13-19
- 25 Years of Recombinant Proteins from Reactor-Grown Cells: Where Do We Go From Here? 2009 Biotechnol Adv
- Manufacturing of Biopharmaceuticals and Implications for Biosimilars 2007 Kidney Blood Press Res. Vol. 30 Suppl 1, pp. 6-8.
- Production of Recombinant Protein Therapeutics in Cultivated Mammalian Cells 2004 Nature Biotechnology 22, 11, 1393-1398.
Publications
- Naming CHO Cells for Bio-manufacturing: Genome Plasticity and Variant Phenotypes of Cell Populations in Bioreactors Question the Relevance of Old Names 2021 Biotechnology Journal, 2021, July, Volume 16, Issue 7; doi.org/10.1002/biot.202100165
- Inducible Overexpression of the Mouse C-myc Protein in Mammalian Cells 1986 Proc Natl Acad Sci USA : 83(15), pp. 5414-5418
- Use of Fluorescence In Situ Hybridisation to Detect and Monitor Transfected and Amplified Sequences in Recombinant CHO Cells 1989 Dev. Biol. Stand., vol. 70, pp. 165-172
- Designing Immunoadhesins for AIDS Therapy 1989 Nature 337 (6207) pp. 525-531
- Review – Integration, Amplification and Stability of Plasmid Sequences in CHO Cell Cultures 1990 Biologicals 18, pp.159-164.
- Integration, Amplification and Stability of Plasmid Sequences in CHO Cell Cultures 1990 Biologicals 18(3): 159-64
- Presence and Transcription of Intracisternal A Particle Related Sequences in CHO Cells 1990 J. Virol, 64 (5), 2021-2032
- Effects of Methotrexate (MTX) on Transfected DNA Stability in Mammalian Cells 1990 Mol Cell Biol, 10 (1) pp. 401-404
- High-Resolution Analysis of Functional Determinants on Human Tissue-Type Plasminogen-Activator 1991 J. Biol Chem 226 (8), pp.5191-5201
- Characterisation of Recombinant CHO Cells 1994 Cell and Tissue Culture: Laboratory Procedures, J.B. Griffiths, A. Doyle, D.G. Newell, Eds., Wiley Interscience
- Plasmid Integration, Amplification and Cytogenetics in CHO Cells 1994 Questions and comments. Biologicals 22, pp. 95-102
- Gene Transfer and Amplification in CHO Cells – Efficient Methods for Maximising Specific Productivity and Assessment of Genetic Consequences 1996 Annals. N.Y. Acad. Sci. 782, pp 70-78
- Transfecting Mammalian Cells: Optimisation of Critical Parameters Affecting Calcium-Phosphate Precipitate Formation 1996 Nucleic Acids Res 24(4), pp. 596-601
- Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells 1997 Genetic Manipulation of Mammalian Cells. Hj. Hauser, R. Wagner, Eds. Walter de Gruyter, pp. 87-120
- Principles of a Scaleable Transient Transfection and Expression Technology for Mammalian Cells 1997 M.J. Carrondo, B. Griffiths and J.P. Moreira: Animal Cell Technology – from Vaccines to Genetic Medicine, pp. 47-50. Kluwer Academic Publishers
- Cytogenetic Characterisation of Recombinant Cells 1998 Methods in Molecular Biology – Mammalian Cell Biology. Nigel Jenkins, Ed. Humana press, pp. 49-59
- Optimizing Production and Recovery of Immunoadhesins 1998 Antibody Fusion Proteins. S. Chamow and A. Ashkenazi, Eds. Wiley and Sons, pp. 281-308
- Establishing and Developing CHO Cell Lines for Commercial Production of Recombinant Human Anti Rh D Antibodies 1999 Animal Cell Technology – Products from Cells, Cells as Products, A Bernard, B Griffiths, W Noe, F Wurm, Eds., Kluwer Acad. Publishers, Dordrecht, pp. 91-94
- Establishing and Developing CHO Cell Lines for Commercial Production of Recombinant Human Anti Rh D Antibodies 1999 Animal Cell Technology – Products from Cells, Cells as Products, A Bernard, B Griffiths, W Noe, F Wurm, Eds., Kluwer Acad. Publishers, Dordrecht, pp. 91-94
- Transient Gene Expression from Mammalian Cells – a New Chapter in Animal Cell Technology? 2001 Cytotechnology 35, 155-156
- Production of Recombinant Protein Therapeutics in Cultivated Mammalian Cells 2004 Nature Biotechnology 22, 11, 1393-1398
- Transfection of Adherent and Suspended Cells by Calcium Phosphate 2004 Methods, Vol. 33, Nr. 2, pp. 136-43
- Tubespin Satellites: a Fast Track Approach for Process Development with Animal Cells Using Shaking Technology 2004 Biochemical Engineering Journal 17 (3), pp. 217-223
- Orbital Shaker Technology for the Cultivation of Mammalian Cells in Suspension 2005 Biotechnol. Bioeng. 89 (4), pp. 400-406
- Surface Wave Dynamics in Orbital Shaken Cylindrical Containers 2014 Physics of Fluids 26, 052104, doi. 10.1063/1.4874612
